Multiplexed Detection of Early Organ Injury Biomarkers in Human Serum Using NanoSPRi-Based Assay

D. Patel, J. M. Obliosca, G. Zhang and Z. Zhou
Luna Innovations Incorporated, Virginia, United States

Keywords: Surface plasmon resonance imaging, organ injury, multiplexed detection, biomarkers in human serum

Identification of biomarkers for early detection of organ injuries such as liver, kidney, heart and lung has been a valuable tool to find preventive and curative strategies needed to reduce morbidity and mortality in critically ill patients. So far detection of organ injury biomarkers relies on methods such as PCR, MS, absorption-based assays, ELISA and microfluidic-Luminex, which are often laborious, expensive and with low specificity and sensitivity. An advanced noninvasive testing with high throughput, high accuracy, fast turnaround time, and cost-effectiveness to early detect specific organ injury is therefore highly desired. To address this issue, Luna is developing a multiplexed biomarker assay based on nanoenhanced SPRi (Surface Plasmon Resonance imaging) technique that is specific and sensitive to a diversity of organ injuries present in human serum. Luna has demonstrated the proposed assay with high sensitivity down to 0.1 pg/mL scale and multiplicity by simultaneous detection of three biomarkers in 10% serum. The success of this project will result in a diagnostic product in helping both military and civilian hospitals determine whether a patient has organ injury, thereby allowing possibility to take early curative intervention.